Measurement

Part:BBa_I2045:Design

Designed by: Bartholomew Canton   Group: iGEM06_MIT   (2007-06-08)


mCherry & GFP expression device for testing a biological virtual machine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1895
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1811


Design Notes

GFP is downstream of mCherry because the e. coli terminator is very efficieint (>95%) and the T7 terminator downstream of GFP is only ~80% efficient.


Source

n/a

References